World Health Organization Publication : Year 1975 ; Issue 9241540486: Guide to the Laboratory Diagnosis of Trachoma

By World Health Organization

Book Id: WPLBN0000125002
Format Type: PDF eBook:
File Size: 2.63 MB
Reproduction Date: 2005

Title:	World Health Organization Publication : Year 1975 ; Issue 9241540486: Guide to the Laboratory Diagnosis of Trachoma
Author:	World Health Organization
Volume:
Language:	English
Subject:	Health., Public health, Wellness programs
Collections:	Medical Library Collection, World Health Collection
Historic Publication Date:
Publisher:	World Health Organization


Citation APA MLA Chicago Health Organization, B. W. (n.d.). World Health Organization Publication : Year 1975 ; Issue 9241540486. Retrieved from http://www.gutenberg.us/

Description
Medical Reference Publication

Excerpt
Recent developments in the laboratory diagnosis of trachoma make it possible to conjirm the clinical diagnosis in a substantial number of cases and to demonstrate the presence of the agent even in the absence of overt clinical signs of active disease. A wider application of these methods would introduce an objective and quantifiable element into diagnosis and epidemiological studies. In order to be able to compare results, ho~~evear , certain degree of uniformity of methods is essential. In consideration of this and of the desirability of sharing some of the experience that has recently been acquired in certain laboratories, a WHO Symposium on the Laboratory Diagnosis of Trachoma was held in London in July 1974, jointly sponsored by the World Health Organization and the Institute of Ophthalmology, University of London.

Table of Contents
CONTENTS Preface . . . . . . . . . . . . . . . . Introduction . . . . . . . . . . . . . . . . . . . . . . . Collection and transportation of specimens . . . . . . . . . Conjunctival specimens for detection of inclusions in smears . Conjunctival specimens for the isolation of Chlamydia . . . . Collection of specimens for the detection of antibodies . . . Detection of inclusions in conjunctival smears . . . . . . . . Giemsa stain . . . . . . . . . . . . . . . . . . . . . . lodine stain . . . . . . . . . . . . . . . . . . . . . . lmmunofluorescent staining for the demonstration of inclusion bodies in conjunctival smears . . . . . . . . . . . . . Choice of staining method for demonstration of inclusions in conjunctival scrapings . . . . . . . . . . . . . . . . . Selected references . . . . . . . . . . . . . . . . . . . Isolation of Chlamydia . . . . . . . Yolk sac method . . . . . . . . . Tissue culture methods . . . . . . Choice of method of isolation . . . Selected references . . . . . . . . Serotyping of the trachoma agent . . . . . . . . . . . . . . Selected references . . . . . . . . . . . . . . . . . . . Serological methods for the laboratory diagnosis of trachoma Complement fixation . . . . . . . . . . . . . . . . . Micro-immunofluorescence (micro-IF) test . . . . . . . Selected references . . . . . . . . . . . . . . . . . .