List of EC numbers (EC 3)

This list contains a list of EC numbers for the third group, EC 3, hydrolases, placed in numerical order as determined by the Nomenclature Committee of the International Union of Biochemistry and Molecular Biology.

EC 3.1: Acting on Ester Bonds 1
- EC 3.1.1: Carboxylic Ester Hydrolases 1.1
- EC 3.1.2: Thioester Hydrolases 1.2
- EC 3.1.3: Phosphoric Monoester Hydrolases 1.3
- EC 3.1.4: Phosphoric Diester Hydrolases 1.4
- EC 3.1.5: Triphosphoric Monoester Hydrolases 1.5
- EC 3.1.6: Sulfuric Ester Hydrolases 1.6
- EC 3.1.7: Diphosphoric Monoester Hydrolases 1.7
- EC 3.1.8: Phosphoric Triester Hydrolases 1.8
- EC 3.1.11: Exodeoxyribonucleases Producing 5'-Phosphomonoesters 1.9
- EC 3.1.13: Exoribonucleases Producing 5'-Phosphomonoesters 1.10
- EC 3.1.14: Exoribonucleases Producing 3'-Phosphomonoesters 1.11
- EC 3.1.15: Exonucleases Active with either Ribo- or Deoxyribonucleic Acids and Producing 5'-Phosphomonoesters 1.12
- EC 3.1.16: Exonucleases Active with either Ribo- or Deoxyribonucleic Acids and Producing 3'-Phosphomonoesters 1.13
- EC 3.1.21: Endodeoxyribonucleases Producing 5'-Phosphomonoesters 1.14
- EC 3.1.22: Endodeoxyribonucleases Producing 3'-Phosphomonoesters 1.15
- EC 3.1.23: and EC 3.1.24 now EC 3.1.21.3, EC 3.1.21.4 and EC 3.1.21.5 1.16
- EC 3.1.25: Site-Specific Endodeoxyribonucleases Specific for Altered Bases 1.17
- EC 3.1.26: Endoribonucleases Producing 5'-Phosphomonoesters 1.18
- EC 3.1.27: Endoribonucleases Producing 3'-Phosphomonoesters 1.19
- EC 3.1.30: Endoribonucleases Active with either Ribo- or Deoxyribonucleic Acids and Producing 5'-Phosphomonoesters 1.20
- EC 3.1.31: Endoribonucleases Active with either Ribo- or Deoxyribonucleic Acids and Producing 3'-Phosphomonoesters 1.21
EC 3.2: Glycosylases 2
- EC 3.2.1: Glycosidase, i.e. enzymes hydrolysing O- and S-glycosyl compounds 2.1
- EC 3.2.2: Hydrolysing N-Glycosyl Compounds 2.2
- EC 3.2.3: Hydrolysing S-Glycosyl Compounds 2.3
EC 3.3: Acting on Ether Bonds 3
- EC 3.3.1: Thioether and trialkylsulfonium hydrolases 3.1
- EC 3.3.2: Ether Hydrolases 3.2
EC 3.4: act on peptide bond - Peptidase 4
- EC 3.4.1 α-amino acyl peptide hydrolases (discontinued) 4.1
- EC 3.4.2 Peptidyl amino acid hydrolases (discontinued) 4.2
- EC 3.4.3 Dipeptide Hydrolases (discontinued) 4.3
- EC 3.4.4 Peptidyl Peptide Hydrolases (discontinued) 4.4
- EC 3.4.11 Aminopeptidase 4.5
- EC 3.4.13 Dipeptidase 4.6
- EC 3.4.14 Dipeptidyl peptidase and tripeptidyl peptidase 4.7
- EC 3.4.15 Peptidyl dipeptidase 4.8
- EC 3.4.16 Serine type carboxypeptidase 4.9
- EC 3.4.17 Metallocarboxypeptidase 4.10
- EC 3.4.18 Cysteine type carboxypeptidase 4.11
- EC 3.4.19 Omega peptidase 4.12
- EC 3.4.21: Serine protease 4.13
- EC 3.4.22 Cysteine protease 4.14
- EC 3.4.23 Aspartic endopeptidase 4.15
- EC 3.4.24: Metallopeptidase 4.16
- EC 3.4.25 Threonine endopeptidase 4.17
- EC 3.4.99 Endopeptidases of unknown catalytic mechanism 4.18
EC 3.5: act on carbon-nitrogen bonds, other than peptide bonds 5
- 3.5.1: linear amides 5.1
- 3.5.2: cyclic amides 5.2
- 3.5.3: linear amidines 5.3
- 3.5.4: cyclic amidines 5.4
- 3.5.5: nitriles 5.5
- 3.5.99: other compounds 5.6
EC 3.6: act on acid anhydride 6
- 3.6.1 6.1
- 3.6.2 6.2
- 3.6.3 6.3
- 3.6.4 6.4
- 3.6.5 6.5
EC 3.7: act on carbon-carbon bonds 7
EC 3.8: act on halide bonds 8
EC 3.9: act on phosphorus-nitrogen bonds 9
EC 3.10: act on sulfur-nitrogen bonds 10
EC 3.11: act on carbon-phosphorus bonds 11
EC 3.12: act on sulfur-sulfur bonds 12
EC 3.13: act on carbon-sulfur bonds 13

EC 3.1: Acting on Ester Bonds

EC 3.1.5: Triphosphoric Monoester Hydrolases

EC 3.1.5.1: dGTPase

EC 3.1.6: Sulfuric Ester Hydrolases

EC 3.1.6.1: arylsulfatase
EC 3.1.6.2: steryl-sulfatase
EC 3.1.6.3: glycosulfatase
EC 3.1.6.4: N-acetylgalactosamine-6-sulfatase
EC 3.1.6.5: deleted
EC 3.1.6.6: choline-sulfatase
EC 3.1.6.7: cellulose-polysulfatase
EC 3.1.6.8: cerebroside-sulfatase
EC 3.1.6.9: chondro-4-sulfatase
EC 3.1.6.10: chondro-6-sulfatase
EC 3.1.6.11: disulfoglucosamine-6-sulfatase
EC 3.1.6.12: N-acetylgalactosamine-4-sulfatase
EC 3.1.6.13: iduronate-2-sulfatase
EC 3.1.6.14: N-acetylglucosamine-6-sulfatase
EC 3.1.6.15: N-sulfoglucosamine-3-sulfatase
EC 3.1.6.16: monomethyl-sulfatase
EC 3.1.6.17: D-lactate-2-sulfatase
EC 3.1.6.18: glucuronate-2-sulfatase

EC 3.1.7: Diphosphoric Monoester Hydrolases

EC 3.1.7.1: prenyl-diphosphatase
EC 3.1.7.2: guanosine-3',5'-bis(diphosphate) 3'-diphosphatase
EC 3.1.7.3: monoterpenyl-diphosphatase
EC 3.1.7.4: sclareol cyclase
EC 3.1.7.5: geranylgeranyl diphosphate diphosphatase
EC 3.1.7.6: farnesyl diphosphatase
EC 3.1.7.7: drimenol cyclase
EC 3.1.7.8: tuberculosinol synthase
EC 3.1.7.9: isotuberculosinol synthase
EC 3.1.7.10: (13E)-labda-7,13-dien-15-ol synthase
EC 3.1.7.11: geranyl diphosphate diphosphatase

EC 3.1.8: Phosphoric Triester Hydrolases

EC 3.1.8.1: aryldialkylphosphatase
EC 3.1.8.2: diisopropyl-fluorophosphatase

EC 3.1.11: Exodeoxyribonucleases Producing 5'-Phosphomonoesters

EC 3.1.11.1: exodeoxyribonuclease I
EC 3.1.11.2: exodeoxyribonuclease III
EC 3.1.11.3: exodeoxyribonuclease (lambda-induced)
EC 3.1.11.4: exodeoxyribonuclease (phage SP3-induced)
EC 3.1.11.5: exodeoxyribonuclease V
EC 3.1.11.6: exodeoxyribonuclease VII

EC 3.1.13: Exoribonucleases Producing 5'-Phosphomonoesters

EC 3.1.13.1: exoribonuclease II
EC 3.1.13.2: exoribonuclease H
EC 3.1.13.3: oligonucleotidase
EC 3.1.13.4: poly(A)-specific ribonuclease
EC 3.1.13.5: ribonuclease D

EC 3.1.14: Exoribonucleases Producing 3'-Phosphomonoesters

EC 3.1.14.1: yeast ribonuclease

EC 3.1.15: Exonucleases Active with either Ribo- or Deoxyribonucleic Acids and Producing 5'-Phosphomonoesters

EC 3.1.15.1: venom exonuclease

EC 3.1.16: Exonucleases Active with either Ribo- or Deoxyribonucleic Acids and Producing 3'-Phosphomonoesters

EC 3.1.16.1: spleen exonuclease

EC 3.1.21: Endodeoxyribonucleases Producing 5'-Phosphomonoesters

EC 3.1.21.1: deoxyribonuclease I
EC 3.1.21.2: deoxyribonuclease IV (phage-T4-induced)
EC 3.1.21.3: type I site-specific deoxyribonuclease
EC 3.1.21.4: type II site-specific deoxyribonuclease
EC 3.1.21.5: type III site-specific deoxyribonuclease
EC 3.1.21.6: CC-preferring endodeoxyribonuclease
EC 3.1.21.7: deoxyribonuclease V

EC 3.1.22: Endodeoxyribonucleases Producing 3'-Phosphomonoesters

EC 3.1.22.1: deoxyribonuclease II
EC 3.1.22.2: Aspergillus deoxyribonuclease K1
EC 3.1.22.3: now EC 3.1.21.7
EC 3.1.22.4: crossover junction endodeoxyribonuclease
EC 3.1.22.5: deoxyribonuclease X

EC 3.1.23: and EC 3.1.24 now EC 3.1.21.3, EC 3.1.21.4 and EC 3.1.21.5

EC 3.1.25: Site-Specific Endodeoxyribonucleases Specific for Altered Bases

EC 3.1.25.1: deoxyribonuclease (pyrimidine dimer)
EC 3.1.25.2: now EC 4.2.99.18

EC 3.1.26: Endoribonucleases Producing 5'-Phosphomonoesters

EC 3.1.26.1: Physarum polycephalum ribonuclease
EC 3.1.26.2: ribonuclease alpha
EC 3.1.26.3: ribonuclease III
EC 3.1.26.4: calf thymus ribonuclease H
EC 3.1.26.5: ribonuclease P
EC 3.1.26.6: ribonuclease IV
EC 3.1.26.7: ribonuclease P4
EC 3.1.26.8: ribonuclease M5
EC 3.1.26.9: ribonuclease (poly-(U)-specific)
EC 3.1.26.10: ribonuclease IX
EC 3.1.26.11: ribonuclease Z
EC 3.1.26.12: ribonuclease E
EC 3.1.26.13: retroviral ribonuclease H
EC 3.1.26.n1: now EC 3.1.26.12

EC 3.1.27: Endoribonucleases Producing 3'-Phosphomonoesters

EC 3.1.27.1: ribonuclease T2
EC 3.1.27.2: Bacillus subtilis ribonuclease
EC 3.1.27.3: ribonuclease T1
EC 3.1.27.4: ribonuclease U2
EC 3.1.27.5: pancreatic ribonuclease
EC 3.1.27.6: Enterobacter ribonuclease
EC 3.1.27.7: ribonuclease F
EC 3.1.27.8: ribonuclease V
EC 3.1.27.9: tRNA-intron endonuclease
EC 3.1.27.10: rRNA endonuclease

EC 3.1.30: Endoribonucleases Active with either Ribo- or Deoxyribonucleic Acids and Producing 5'-Phosphomonoesters

EC 3.1.30.1: Aspergillus nuclease S1
EC 3.1.30.2: Serratia marcescens nuclease

EC 3.1.31: Endoribonucleases Active with either Ribo- or Deoxyribonucleic Acids and Producing 3'-Phosphomonoesters

EC 3.1.31.1: micrococcal nuclease

EC 3.2: Glycosylases

EC 3.2.1: Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds

EC 3.2.1.1: α-amylase
EC 3.2.1.2: β-amylase
EC 3.2.1.3: glucan 1,4-a-glucosidase
EC 3.2.1.4: cellulase
EC 3.2.1.5: deleted
EC 3.2.1.6: endo-1,3(4)-b-glucanase
EC 3.2.1.7: inulinase
EC 3.2.1.8: endo-1,4-β-xylanase
EC 3.2.1.9: deleted
EC 3.2.1.10: oligo-1,6-glucosidase
EC 3.2.1.11: dextranase
EC 3.2.1.12: deleted, included in EC 3.2.1.54
EC 3.2.1.13: deleted, included in EC 3.2.1.54
EC 3.2.1.14: chitinase
EC 3.2.1.15: polygalacturonase
EC 3.2.1.16: deleted
EC 3.2.1.17: lysozyme
EC 3.2.1.18: exo-a-sialidase
EC 3.2.1.19: deleted
EC 3.2.1.20: a-glucosidase
EC 3.2.1.21: b-glucosidase
EC 3.2.1.22: α-galactosidase
EC 3.2.1.23: b-galactosidase
EC 3.2.1.24: a-mannosidase
EC 3.2.1.25: b-mannosidase
EC 3.2.1.26: b-fructofuranosidase
EC 3.2.1.27: deleted
EC 3.2.1.28: α,α-trehalase
EC 3.2.1.29: deleted, included in EC 3.2.1.52
EC 3.2.1.30: deleted, included in EC 3.2.1.52
EC 3.2.1.31: β-glucuronidase
EC 3.2.1.32: xylan endo-1,3-b-xylosidase
EC 3.2.1.33: amylo-1,6-glucosidase
EC 3.2.1.34: deleted, included in EC 3.2.1.35
EC 3.2.1.35: hyaluronoglucosaminidase
EC 3.2.1.36: hyaluronoglucuronidase
EC 3.2.1.37: xylan 1,4-b-xylosidase
EC 3.2.1.38: b-D-fucosidase
EC 3.2.1.39: glucan endo-1,3-b-D-glucosidase
EC 3.2.1.40: a-L-rhamnosidase
EC 3.2.1.41: pullulanase
EC 3.2.1.42: GDP-glucosidase
EC 3.2.1.43: b-L-rhamnosidase
EC 3.2.1.44: fucoidanase
EC 3.2.1.45: glucosylceramidase
EC 3.2.1.46: galactosylceramidase
EC 3.2.1.47: galactosylgalactosylglucosylceramidase
EC 3.2.1.48: Sucrose alpha-glucosidase
EC 3.2.1.49: a-N-acetylgalactosaminidase
EC 3.2.1.50: α-N-acetylglucosaminidase
EC 3.2.1.51: a-L-fucosidase
EC 3.2.1.52: b-L-N-acetylhexosaminidase
EC 3.2.1.53: b-N-acetylgalactosaminidase
EC 3.2.1.54: cyclomaltodextrinase
EC 3.2.1.55: a-N-arabinofuranosidase
EC 3.2.1.56: glucuronosyl-disulfoglucosamine glucuronidase
EC 3.2.1.57: isopullulanase
EC 3.2.1.58: glucan 1,3-b-glucosidase
EC 3.2.1.59: glucan endo-1,3-a-glucosidase
EC 3.2.1.60: glucan 1,4-a-maltotetraohydrolase
EC 3.2.1.61: mycodextranase
EC 3.2.1.62: glycosylceramidase
EC 3.2.1.63: 1,2-alpha-L-fucosidase
EC 3.2.1.64: 2,6-b-fructan 6-levanbiohydrolase
EC 3.2.1.65: levanase
EC 3.2.1.66: quercitrinase
EC 3.2.1.67: galacturan 1,4-a-galacturonidase
EC 3.2.1.68: isoamylase
EC 3.2.1.69: deleted, included in EC 3.2.1.41
EC 3.2.1.70: glucan 1,6-a-glucosidase
EC 3.2.1.71: glucan endo-1,2-b-glucosidase
EC 3.2.1.72: xylan 1,3-b-xylosidase
EC 3.2.1.73: licheninase
EC 3.2.1.74: glucan 1,4-b-glucosidase
EC 3.2.1.75: glucan endo-1,6-b-glucosidase
EC 3.2.1.76: L-iduronidase
EC 3.2.1.77: mannan 1,2-(1,3)-a-mannosidase
EC 3.2.1.78: mannan endo-1,4-b-mannosidase
EC 3.2.1.79: deleted, included in EC 3.2.1.55
EC 3.2.1.80: fructan b-fructosidase
EC 3.2.1.81: agarase
EC 3.2.1.82: exo-poly-a-galacturonosidase
EC 3.2.1.83: k-carrageenase
EC 3.2.1.84: glucan 1,3-a-glucosidase
EC 3.2.1.85: 6-phospho-b-galactosidase
EC 3.2.1.86: 6-phospho-b-glucosidase
EC 3.2.1.87: capsular-polysaccharide endo-1,3-a-galactosidase
EC 3.2.1.88: b-L-arabinosidase
EC 3.2.1.89: arabinogalactan endo-1,4-b-galactosidase
EC 3.2.1.90: Deleted, not sufficiently characterised.
EC 3.2.1.91: cellulose 1,4-b-cellobiosidase
EC 3.2.1.92: peptidoglycan b-N-acetylmuramidase
EC 3.2.1.93: a,a-phosphotrehalase
EC 3.2.1.94: glucan 1,6-a-isomaltosidase
EC 3.2.1.95: dextran 1,6-a-isomaltotriosidase
EC 3.2.1.96: mannosyl-glycoprotein endo-b-N-acetylglucosaminidase
EC 3.2.1.97: glycopeptide a-N-acetylgalactosaminidase
EC 3.2.1.98: glucan 1,4-a-maltohexaosidase
EC 3.2.1.99: arabinan endo-1,5-a-L-arabinosidase
EC 3.2.1.100: mannan 1,4-mannobiosidase
EC 3.2.1.101: mannan endo-1,6-a-mannosidase
EC 3.2.1.102: blood-group-substance endo-1,4-b-galactosidase
EC 3.2.1.103: keratan-sulfate endo-1,4-b-galactosidase
EC 3.2.1.104: steryl-b-glucosidase
EC 3.2.1.105: strictosidine b-glucosidase
EC 3.2.1.106: mannosyl-oligosaccharide glucosidase
EC 3.2.1.107: protein-glucosylgalactosylhydroxylysine glucosidase
EC 3.2.1.108: lactase
EC 3.2.1.109: endogalactosaminidase
EC 3.2.1.110: mucinaminylserine mucinaminidase
EC 3.2.1.111: 1,3-alpha-L-fucosidase
EC 3.2.1.112: 2-deoxyglucosidase
EC 3.2.1.113: mannosyl-oligosaccharide 1,2-a-mannosidase
EC 3.2.1.114: mannosyl-oligosaccharide 1,3-1,6-a-mannosidase
EC 3.2.1.115: branched-dextran exo-1,2-a-glucosidase
EC 3.2.1.116: glucan 1,4-a-maltotriohydrolase
EC 3.2.1.117: amygdalin b-glucosidase
EC 3.2.1.118: prunasin b-glucosidase
EC 3.2.1.119: vicianin b-glucosidase
EC 3.2.1.120: oligoxyloglucan b-glycosidase
EC 3.2.1.121: polymannuronate hydrolase
EC 3.2.1.122: maltose-6'-phosphate glucosidase
EC 3.2.1.123: endoglycosylceramidase
EC 3.2.1.124: 3-deoxy-2-octulosonidase
EC 3.2.1.125: raucaffricine b-glucosidase
EC 3.2.1.126: coniferin b-glucosidase
EC 3.2.1.127: 1,6-alpha-L-fucosidase
EC 3.2.1.128: glycyrrhizinate beta-glucuronidase
EC 3.2.1.129: endo-a-sialidase
EC 3.2.1.130: glycoprotein endo-a-1,2-mannosidase
EC 3.2.1.131: xylan a-1,2-glucuronosidase
EC 3.2.1.132: chitosanase
EC 3.2.1.133: glucan 1,4-a-maltohydrolase
EC 3.2.1.134: difructose-anhydride synthase
EC 3.2.1.135: neopullulanase
EC 3.2.1.136: glucuronoarabinoxylan endo-1,4-b-xylanase
EC 3.2.1.137: mannan exo-1,2-1,6-a-mannosidase
EC 3.2.1.138: now EC 4.2.2.15
EC 3.2.1.139: Alpha-glucuronidase
EC 3.2.1.140: lacto-N-biosidase
EC 3.2.1.141: 4-a-D-((1-4)-a-D-glucano)trehalose trehalohydrolase
EC 3.2.1.142: limit dextrinase
EC 3.2.1.143: poly(ADP-ribose) glycohydrolase
EC 3.2.1.144: 3-deoxyoctulosonase
EC 3.2.1.145: galactan 1,3-b-galactosidase
EC 3.2.1.146: b-galactofuranosidase
EC 3.2.1.147: thioglucosidase
EC 3.2.1.148: now EC 4.4.1.21
EC 3.2.1.149: b-primeverosidase
EC 3.2.1.150: oligoxyloglucan reducing-end-specific cellobiohydrolase
EC 3.2.1.151: xyloglucan-specific endo-b-1,4-glucanase
EC 3.2.1.152: mannosylglycoprotein endo-b-mannosidase
EC 3.2.1.153: fructan b-(2,1)-fructosidase
EC 3.2.1.154: fructan b-(2,6)-fructosidase
EC 3.2.1.155: xyloglucan-specific exo-beta-1,4-glucanase
EC 3.2.1.156: oligosaccharide reducing-end xylanase
EC 3.2.1.157: iota-carrageenase
EC 3.2.1.158: alpha-agarase
EC 3.2.1.159: alpha-neoagaro-oligosaccharide hydrolase
EC 3.2.1.160: now EC 3.2.1.155
EC 3.2.1.161: beta-apiosyl-beta-glucosidase
EC 3.2.1.162: lambda-carrageenase
EC 3.2.1.163: 1,6-alpha-D-mannosidase
EC 3.2.1.164: galactan endo-1,6-beta-galactosidase
EC 3.2.1.165: exo-1,4-beta-D-glucosaminidase
EC 3.2.1.166: blood group B branched chain alpha-1,3-galactosidase
EC 3.2.1.167: blood group B linear chain alpha-1,3-galactosidase
EC 3.2.1.168: hesperidin 6-O-alpha-L-rhamnosyl-beta-D-glucosidase
EC 3.2.1.169: protein O-GlcNAcase
EC 3.2.1.170: mannosylglycerate hydrolase
EC 3.2.1.171: rhamnogalacturonan hydrolase
EC 3.2.1.172: unsaturated rhamnogalacturonyl hydrolase
EC 3.2.1.173: rhamnogalacturonan galacturonohydrolase
EC 3.2.1.174: rhamnogalacturonan rhamnohydrolase
EC 3.2.1.175: beta-D-glucopyranosyl abscisate beta-glucosidase
EC 3.2.1.176: cellulose 1,4-beta-cellobiosidase (reducing end)
EC 3.2.1.177: alpha-D-xyloside xylohydrolase
EC 3.2.1.178: beta-porphyranase
EC 3.2.1.179: gellan tetrasaccharide unsaturated glucuronyl hydrolase
EC 3.2.1.180: unsaturated chondroitin disaccharide hydrolase
EC 3.2.1.181: galactan endo-beta-1,3-galactanase
EC 3.2.1.182: 4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl glucoside beta-D-glucosidase
EC 3.2.1.183: UDP-N-acetylglucosamine 2-epimerase (hydrolysing)
EC 3.2.1.184: UDP-N,N'-diacetylbacillosamine 2-epimerase (hydrolysing)

EC 3.2.2: Hydrolysing N-Glycosyl Compounds

EC 3.2.2.1: purine nucleosidase
EC 3.2.2.2: inosine nucleosidase
EC 3.2.2.3: uridine nucleosidase
EC 3.2.2.4: AMP nucleosidase
EC 3.2.2.5: NAD+ nucleosidase
EC 3.2.2.6: NAD(P)+ nucleosidase
EC 3.2.2.7: adenosine nucleosidase
EC 3.2.2.8: ribosylpyrimidine nucleosidase
EC 3.2.2.9: adenosylhomocysteine nucleosidase
EC 3.2.2.10: pyrimidine-5'-nucleotide nucleosidase
EC 3.2.2.11: b-aspartyl-N-acetylglucosaminidase
EC 3.2.2.12: inosinate nucleosidase
EC 3.2.2.13: 1-methyladenosine nucleosidase
EC 3.2.2.14: NMN nucleosidase
EC 3.2.2.15: DNA-deoxyinosine glycosylase
EC 3.2.2.16: methylthioadenosine nucleosidase
EC 3.2.2.17: deoxyribodipyrimidine endonucleosidase
EC 3.2.2.18: deleted, included in EC 3.5.1.52
EC 3.2.2.19: ADP-ribosylarginine hydrolase
EC 3.2.2.20: DNA-3-methyladenine glycosylase I
EC 3.2.2.21: DNA-3-methyladenine glycosylase II
EC 3.2.2.22: rRNA N-glycosylase
EC 3.2.2.23: DNA-formamidopyrimidine glycosylase
EC 3.2.2.24: ADP-ribosyl-(dinitrogen reductase) hydrolase
EC 3.2.2.25: N-methyl nucleosidase
EC 3.2.2.26: futalosine hydrolase
EC 3.2.2.27: uracil-DNA glycosylase
EC 3.2.2.28: double-stranded uracil-DNA glycosylase
EC 3.2.2.29: thymine-DNA glycosylase

EC 3.2.3: Hydrolysing S-Glycosyl Compounds

EC 3.2.3.1: now EC 3.2.1.147

EC 3.3: Acting on Ether Bonds

EC 3.3.1: Thioether and trialkylsulfonium hydrolases

EC 3.3.1.1: adenosylhomocysteinase
EC 3.3.1.2: adenosylmethionine hydrolase
EC 3.3.1.3: now EC 3.2.1.148

EC 3.3.2: Ether Hydrolases

EC 3.3.2.1: isochorismatase
EC 3.3.2.2: alkenylglycerophosphocholine hydrolase
EC 3.3.2.3: epoxide hydrolase
EC 3.3.2.4: trans-epoxysuccinate hydrolase
EC 3.3.2.5: alkenylglycerophosphoethanolamine hydrolase
EC 3.3.2.6: leukotriene-A4 hydrolase
EC 3.3.2.7: hepoxilin-epoxide hydrolase
EC 3.3.2.8: limonene-1,2-epoxide hydrolase
EC 3.3.2.9: microsomal epoxide hydrolase
EC 3.3.2.10: soluble epoxide hydrolase
EC 3.3.2.11: cholesterol-5,6-oxide hydrolase

EC 3.4: act on peptide bonds - Peptidase

EC 3.4.1 α-amino acyl peptide hydrolases (discontinued)

EC 3.4.1.1: leucine aminopeptidase, now EC 3.4.11.1
EC 3.4.1.2: alanine aminopeptidase, now EC 3.4.11.2
EC 3.4.1.3: aminotripeptidase, now EC 3.4.11.4
EC 3.4.1.4: proline iminopeptidase, now EC 3.4.11.5

EC 3.4.2 Peptidyl amino acid hydrolases (discontinued)

EC 3.4.2.1: carboxypeptidase A, now EC 3.4.17.1
EC 3.4.2.2: carboxypeptidase B, now EC 3.4.17.2
EC 3.4.2.3: yeast carboxypeptidase, now EC 3.4.17.4

EC 3.4.3 Dipeptide Hydrolases (discontinued)

EC 3.4.3.1: glycyl-glycine dipeptidase, now EC 3.4.13.18
EC 3.4.3.2: glycyl-leucine dipeptidase, now EC 3.4.13.18
EC 3.4.3.3: aminoacyl-histidine dipeptidase, carnosinase, now EC 3.4.13.3
EC 3.4.3.4: aminoacyl-methylhistidine dipeptidase, anserinase, now EC 3.4.13.5
EC 3.4.3.5: cysteinyl-glycine dipeptidase, now EC 3.4.13.6
EC 3.4.3.6: iminodipeptidase, now EC 3.4.13.8
EC 3.4.3.7: imidodipeptidase, prolidase, now EC 3.4.13.9

EC 3.4.4 Peptidyl Peptide Hydrolases (discontinued)

EC 3.4.4.1: pepsin, now EC 3.4.23.1
EC 3.4.4.2: pepsin B, now EC 3.4.23.2
EC 3.4.4.3: rennin, now EC 3.4.23.4
EC 3.4.4.4: trypsin, now EC 3.4.21.4
EC 3.4.4.5: chymotrypsin, now EC 3.4.21.1
EC 3.4.4.6: chymotrypsin B, now EC 3.4.21.1
EC 3.4.4.7: pancreatopeptidase E, now EC 3.4.21.36 and EC 3.4.21.37
EC 3.4.4.8: enteropeptidase, now EC 3.4.21.9
EC 3.4.4.9: cathepsin C, now EC 3.4.14.1
EC 3.4.4.10: papain now EC 3.4.22.2
EC 3.4.4.11: chymopapain, now EC 3.4.22.6
EC 3.4.4.12: ficin, now EC 3.4.22.3
EC 3.4.4.13: thrombin, now EC 3.4.21.5
EC 3.4.4.14: plasmin, now EC 3.4.21.7
EC 3.4.4.15: renin, now EC 3.4.23.15
EC 3.4.4.16: subtilopeptidase A, now EC 3.4.21.62 to EC 3.4.21.67
EC 3.4.4.17: aspergillopeptidase A, now EC 3.4.23.20 to EC 3.4.23.30 3.4.23.30
EC 3.4.4.18: streptococcus peptidase A, now EC 3.4.22.10
EC 3.4.4.19: clostridiopeptidase A, now EC 3.4.24.3
EC 3.4.4.20: clostridiopeptidase B, now EC 3.4.22.8
EC 3.4.4.21: kallikrein, now EC 3.4.21.34
EC 3.4.4.22: recommended Name never specified, now EC 3.4.23.3
EC 3.4.4.23: recommended Name never specified, now EC 3.4.23.5
EC 3.4.4.24: recommended Name never specified, now EC 3.4.22.32 and EC 3.4.22.33
EC 3.4.4.25: keratinase deleted

EC 3.4.11 Aminopeptidases

EC 3.4.11.1: leucyl aminopeptidase
EC 3.4.11.2: membrane alanyl aminopeptidase
EC 3.4.11.3: cystinyl aminopeptidase
EC 3.4.11.4: tripeptide aminopeptidase
EC 3.4.11.5: prolyl aminopeptidase
EC 3.4.11.6: aminopeptidase B
EC 3.4.11.7: glutamyl aminopeptidase
EC 3.4.11.8: Transferred entry: pyroglutamyl aminopeptidase. Now EC 3.4.19.3, pyroglutamyl-peptidase I
EC 3.4.11.9: X-Pro aminopeptidase
EC 3.4.11.10: bacterial leucyl aminopeptidase
EC 3.4.11.11: Deleted entry: aminopeptidase
EC 3.4.11.12: Deleted entry: thermophilic aminopeptidase
EC 3.4.11.13: Clostridial aminopeptidase
EC 3.4.11.14: cytosol alanyl aminopeptidase
EC 3.4.11.15: aminopeptidase Y
EC 3.4.11.16: X-Trp aminopeptidase
EC 3.4.11.17: tryptophanyl aminopeptidase
EC 3.4.11.18: methionyl aminopeptidase
EC 3.4.11.19: D-stereospecific aminopeptidase
EC 3.4.11.20: aminopeptidase Ey
EC 3.4.11.21: aspartyl aminopeptidase
EC 3.4.11.22: aminopeptidase I
EC 3.4.11.23: PepB aminopeptidase
EC 3.4.11.24: aminopeptidase S
EC 3.4.11.25: beta-peptidyl aminopeptidase
EC 3.4.11.26: intermediate cleaving peptidase 55

EC 3.4.13 Dipeptidases

EC 3.4.13.1: glycyl-glycine dipeptidase, now EC 3.4.13.18
EC 3.4.13.2: glycyl-leucine dipeptidase, now EC 3.4.13.18
EC 3.4.13.3: X-His dipeptidase
EC 3.4.13.4: X-Arg dipeptidase
EC 3.4.13.5: X-methyl-His dipeptidase
EC 3.4.13.6: Transferred entry: Cys-Gly dipeptidase. Now EC 3.4.11.2, membrane alanyl aminopeptidase
EC 3.4.13.7: Glu-Glu dipeptidase
EC 3.4.13.8: Transferred entry: Pro-X dipeptidase. Now EC 3.4.13.18, cytosol nonspecific dipeptidase
EC 3.4.13.9: X-Pro dipeptidase
EC 3.4.13.10: Transferred entry: β-aspartyldipeptidase. Now EC 3.4.19.5, β-aspartyl-peptidase
EC 3.4.13.11: Transferred entry: dipeptidase. Now EC 3.4.13.19, membrane dipeptidase
EC 3.4.13.12: Met-X dipeptidase
EC 3.4.13.13: Transferred entry: homocarnosinase. Now EC 3.4.13.3, X-His dipeptidase
EC 3.4.13.14: Deleted entry: γ-glutamyldipeptidase
EC 3.4.13.15: Transferred entry: N2-β-alanylarginine dipeptidase. Now EC 3.4.13.18, cytosol nonspecific dipeptidase
EC 3.4.13.16: Deleted entry: aspartylphenylalanine dipeptidase
EC 3.4.13.17: non-stereospecific dipeptidase
EC 3.4.13.18: cytosol nonspecific dipeptidase
EC 3.4.13.19: membrane dipeptidase
EC 3.4.13.20: beta-Ala-His dipeptidase
EC 3.4.13.21: dipeptidase E
EC 3.4.13.22: D-Ala-D-Ala dipeptidase

EC 3.4.14 Dipeptidyl peptidases and tripeptidyl peptidases

EC 3.4.14.1: dipeptidyl-peptidase I
EC 3.4.14.2: dipeptidyl-peptidase II
EC 3.4.14.3: Transferred entry: acylamino-acid-releasing enzyme. Now EC 3.4.19.1, acylaminoacyl-peptidase
EC 3.4.14.4: dipeptidyl-peptidase III
EC 3.4.14.5: dipeptidyl-peptidase IV
EC 3.4.14.6: dipeptidyl-dipeptidase
EC 3.4.14.7: Deleted entry: tetralysine endopeptidase
EC 3.4.14.8: Transferred entry: tripeptidyl peptidase. Now EC 3.4.14.10, tripeptidyl-peptidase II
EC 3.4.14.9: tripeptidyl-peptidase I
EC 3.4.14.10: tripeptidyl-peptidase II
EC 3.4.14.11: Xaa-Pro dipeptidyl-peptidase
EC 3.4.14.12: Xaa-Xaa-Pro tripeptidyl-peptidase

EC 3.4.15 Peptidyl dipeptidases

EC 3.4.15.1: peptidyl-dipeptidase A
EC 3.4.15.2: Transferred entry: pepdidyl carboxyamidase. Now EC 3.4.19.2, peptidyl-glycinamidase
EC 3.4.15.3: Transferred entry: dipeptidyl carboxypeptidase. Now EC 3.4.15.5, peptidyl-dipeptidase Dcp
EC 3.4.15.4: Peptidyl-dipeptidase B
EC 3.4.15.5: Peptidyl-dipeptidase Dcp
EC 3.4.15.6: cyanophycinase

EC 3.4.16 Serine type carboxypeptidases

EC 3.4.16.1: Transferred entry: serine carboxypeptidase. Now EC 3.4.16.6, carboxypeptidase D
EC 3.4.16.2: lysosomal Pro-X carboxypeptidase
EC 3.4.16.3: Transferred entry: tyrosine carboxypeptidase. Now included with EC 3.4.16.5, carboxypeptidase C
EC 3.4.16.4: serine-type D-Ala-D-Ala carboxypeptidase
EC 3.4.16.5: carboxypeptidase c
EC 3.4.16.6: carboxypeptidase D

EC 3.4.17 Metallocarboxypeptidases

EC 3.4.17.1: carboxypeptidase A
EC 3.4.17.2: carboxypeptidase B
EC 3.4.17.3: lysine carboxypeptidase
EC 3.4.17.4: Gly-X carboxypeptidase
EC 3.4.17.5: Deleted entry: aspartate carboxypeptidase
EC 3.4.17.6: alanine carboxypeptidase
EC 3.4.17.7: Transferred entry: acylmuramoyl-alanine carboxypeptidase. Now EC 3.5.1.28, N-acetylmuramoyl-L-alanine amidase
EC 3.4.17.8: muramoylpentapeptide carboxypeptidase
EC 3.4.17.9: Transferred entry: carboxypeptidase S. Now included with EC 3.4.17.4, Gly-Xaa carboxypeptidase
EC 3.4.17.10: carboxypeptidase E
EC 3.4.17.11: glutamate carboxypeptidase
EC 3.4.17.12: carboxypeptidase M
EC 3.4.17.13: Muramoyltetrapeptide carboxypeptidase
EC 3.4.17.14: Zinc D-Ala-D-Ala carboxypeptidase
EC 3.4.17.15: carboxypeptidase A2
EC 3.4.17.16: Membrane Pro-X carboxypeptidase
EC 3.4.17.17: tubulinyl-Tyr carboxypeptidase
EC 3.4.17.18: carboxypeptidase T
EC 3.4.17.19: Carboxypeptidase Taq
EC 3.4.17.20: Carboxypeptidase u
EC 3.4.17.21: Glutamate carboxypeptidase II
EC 3.4.17.22: Metallocarboxypeptidase D
EC 3.4.17.23: angiotensin-converting enzyme 2

EC 3.4.18 Cysteine type carboxypeptidases

EC 3.4.18.1: cathepsin X

EC 3.4.19 Omega peptidases

EC 3.4.19.1: acylaminoacyl-peptidase
EC 3.4.19.2: peptidyl-glycinamidase
EC 3.4.19.3: pyroglutamyl-peptidase I
EC 3.4.19.4: Deleted entry: N-acetylmethionylpeptide peptidase
EC 3.4.19.5: beta-aspartyl-peptidase
EC 3.4.19.6: pyroglutamyl-peptidase II
EC 3.4.19.7: N-Formylmethionyl-peptidase
EC 3.4.19.8: Transferred entry: now EC 3.4.17.21, glutamate carboxypeptidase II
EC 3.4.19.9: gamma-glutamyl hydrolase
EC 3.4.19.10: Transferred entry: acylmuramoyl-Ala peptidase. Now EC 3.5.1.28, N-acetylmuramoyl-L-alanine amidase
EC 3.4.19.11: gamma-D-Glutamyl-meso-diaminopimelate peptidase
EC 3.4.19.12: ubiquitinyl hydrolase 1
EC 3.4.19.13: glutathione hydrolase
EC 3.4.19.14: leukotriene-C4 hydrolase

EC 3.4.21: Serine proteases

EC 3.4.21.1: chymotrypsin
EC 3.4.21.2: chymotrypsin C
EC 3.4.21.3: metridin
EC 3.4.21.4: trypsin
EC 3.4.21.5: thrombin
EC 3.4.21.6: coagulation factor Xa
EC 3.4.21.7: plasmin
EC 3.4.21.8: Transferred entry: kallikrein. Now EC 3.4.21.34 (plasma kallikrein) and EC 3.4.21.35 (tissue kallikrein)
EC 3.4.21.9: enteropeptidase
EC 3.4.21.10: acrosin
EC 3.4.21.11: Transferred entry: elastase. Now EC 3.4.21.37, leukocyte elastase
EC 3.4.21.12: alpha-lytic endopeptidase
EC 3.4.21.13: Transferred entry: Phaseolus proteinase. Now EC 3.4.16.6, carboxypeptidase D
EC 3.4.21.14: Transferred entry: now EC 3.4.21.67 endopeptidase So
EC 3.4.21.15: Transferred entry: Aspergillus alkaline proteinase. Now EC 3.4.21.63, oryzin
EC 3.4.21.16: Deleted entry: Alternaria serine proteinase
EC 3.4.21.17: Deleted entry: Arthrobacter serine proteinase
EC 3.4.21.18: Deleted entry: Tenebrio α-proteinase
EC 3.4.21.19: glutamyl endopeptidase
EC 3.4.21.20: cathepsin G
EC 3.4.21.21: coagulation factor VIIa
EC 3.4.21.22: coagulation factor IXa
EC 3.4.21.23: Deleted entry: Vipera russelli proteinase
EC 3.4.21.24: Deleted entry: red cell neutral endopeptidase
EC 3.4.21.25: cucumisin
EC 3.4.21.26: prolyl oligopeptidase
EC 3.4.21.27: coagulation factor XIa
EC 3.4.21.28: Transferred entry: Agkistrodon serine proteinase. Now EC 3.4.21.74, venombin A
EC 3.4.21.29: Transferred entry: Bothrops atrox serine proteinase. Now EC 3.4.21.74, venombin A
EC 3.4.21.30: Transferred entry: Crotalus adamanteus serine proteinase. Now EC 3.4.21.74, venombin A
EC 3.4.21.31: Transferred entry: urokinase. Now EC 3.4.21.73, u-plasminogen activator
EC 3.4.21.32: brachyurin
EC 3.4.21.33: Deleted entry: Entomophthora collagenolytic proteinase
EC 3.4.21.34: plasma kallikrein
EC 3.4.21.35: renal tissue kallikrein
EC 3.4.21.36: pancreatic elastase
EC 3.4.21.37: leukocyte elastase
EC 3.4.21.38: coagulation factor XIIa
EC 3.4.21.39: chymase
EC 3.4.21.40: Deleted entry: submandibular proteinase A
EC 3.4.21.41: complement subcomponent C1r
EC 3.4.21.42: complement subcomponent C1s
EC 3.4.21.43: classical-complement-pathway C3/C5 convertase
EC 3.4.21.44: Transferred entry: complement component C5 convertase. Now EC 3.4.21.43, classical-complement-pathway C3/C5 convertase
EC 3.4.21.45: complement factor I
EC 3.4.21.46: complement factor D
EC 3.4.21.47: alternative-complement-pathway C3/C5 convertase
EC 3.4.21.48: cerevisin
EC 3.4.21.49: hypodermin C
EC 3.4.21.50: lysyl endopeptidase
EC 3.4.21.51: Deleted entry: Leukocyte-membrane neutral endopeptidase
EC 3.4.21.52: Deleted entry: Cathepsin R
EC 3.4.21.53: Endopeptidase La
EC 3.4.21.54: gamma-Renin
EC 3.4.21.55: Venombin AB
EC 3.4.21.56: Deleted entry: euphorbain. Now considered EC 3.4.21.25, cucumisin
EC 3.4.21.57: Leucyl endopeptidase
EC 3.4.21.58: Deleted entry: prohormone serine proteinase
EC 3.4.21.59: Tryptase
EC 3.4.21.60: Scutelarin
EC 3.4.21.61: Kexin
EC 3.4.21.62: Subtilisin
EC 3.4.21.63: Oryzin
EC 3.4.21.64: Endopeptidase K
EC 3.4.21.65: Thermomycolin
EC 3.4.21.66: Thermitase
EC 3.4.21.67: Endopeptidase So
EC 3.4.21.68: t-Plasminogen activator
EC 3.4.21.69: Protein C (activated)
EC 3.4.21.70: Pancreatic endopeptidase E
EC 3.4.21.71: Pancreatic elastase II
EC 3.4.21.72: IgA-specific serine endopeptidase
EC 3.4.21.73: u-Plasminogen activator
EC 3.4.21.74: Venombin A
EC 3.4.21.75: Furin
EC 3.4.21.76: Myeloblastin
EC 3.4.21.77: semenogelase
EC 3.4.21.78: Granzyme A
EC 3.4.21.79: Granzyme B
EC 3.4.21.80: Streptogrisin A
EC 3.4.21.81: Streptogrisin B
EC 3.4.21.82: Glutamyl endopeptidase II
EC 3.4.21.83: Oligopeptidase B
EC 3.4.21.84: Limulus clotting factor C
EC 3.4.21.85: Limulus clotting factor B
EC 3.4.21.86: Limulus clotting enzyme
EC 3.4.21.87: Transferred entry: omptin. Now EC 3.4.23.49, omptin. The enzyme is not a serine protease, as thought previously, but an aspartate protease
EC 3.4.21.88: Repressor LexA
EC 3.4.21.89: Signal peptidase I
EC 3.4.21.90: Togavirin
EC 3.4.21.91: Flavivirin
EC 3.4.21.92: Endopeptidase Clp
EC 3.4.21.93: Proprotein convertase 1
EC 3.4.21.94: Proprotein convertase 2
EC 3.4.21.95: Snake venom factor V activator
EC 3.4.21.96: Lactocepin
EC 3.4.21.97: assemblin
EC 3.4.21.98: hepacivirin
EC 3.4.21.99: spermosin
EC 3.4.21.100: pseudomonalisin
EC 3.4.21.101: xanthomonalisin
EC 3.4.21.102: C-terminal processing peptidase
EC 3.4.21.103: physarolisin
EC 3.4.21.104: mannan-binding lectin-associated serine protease-2
EC 3.4.21.105: rhomboid protease
EC 3.4.21.106: hepsin
EC 3.4.21.107: peptidase Do
EC 3.4.21.108: HtrA2 peptidase
EC 3.4.21.109: matriptase
EC 3.4.21.110: C5a peptidase
EC 3.4.21.111: aqualysin 1
EC 3.4.21.112: site-1 protease
EC 3.4.21.113: pestivirus NS3 polyprotein peptidase
EC 3.4.21.114: equine arterivirus serine peptidase
EC 3.4.21.115: infectious pancreatic necrosis birnavirus Vp4 peptidase
EC 3.4.21.116: SpoIVB peptidase
EC 3.4.21.117: stratum corneum chymotryptic enzyme
EC 3.4.21.118: kallikrein 8
EC 3.4.21.119: kallikrein 13
EC 3.4.21.120: oviductin

EC 3.4.22 Cysteine proteases

EC 3.4.22.1: cathepsin B
EC 3.4.22.2: papain
EC 3.4.22.3: ficain
EC 3.4.22.4: bromelain (stem). Now EC 3.4.22.32 (stem bromelain) and EC 3.4.22.33 (fruit bromelain)
EC 3.4.22.5: bromelain (juice). Now EC 3.4.22.32 (stem bromelain) and EC 3.4.22.33 (fruit bromelain)
EC 3.4.22.6: chymopapain
EC 3.4.22.7: asclepain
EC 3.4.22.8: clostripain
EC 3.4.22.9: yeast proteinase B. Now EC 3.4.21.48, cerevisin
EC 3.4.22.10: streptopain
EC 3.4.22.11: insulinase. Now EC 3.4.24.56, insulysin
EC 3.4.22.12: gamma-glutamyl hydrolase. Now EC 3.4.19.9, γ-glutamyl hydrolase
EC 3.4.22.13: Deleted entry: staphylococcal cysteine proteinase
EC 3.4.22.14: actinidain
EC 3.4.22.15: cathepsin L
EC 3.4.22.16: cathepsin H
EC 3.4.22.17: Transferred entry: calpain. Now EC 3.4.22.53, calpain-2
EC 3.4.22.18: Transferred entry: prolyl endopeptidase (thiol-dependent). Now EC 3.4.21.26, prolyl oligopeptidase
EC 3.4.22.19: Transferred entry: endo-oligopeptidase. Now EC 3.4.24.15, thimet oligopeptidase
EC 3.4.22.20: Deleted entry: dinorphin-converting enzyme
EC 3.4.22.21: Transferred entry: yeast cysteine proteinase E. Now EC 3.4.25.1, proteasome endopeptidase complex
EC 3.4.22.22: Transferred entry: yeast cysteine proteinase D. Now EC 3.4.24.37, saccharolysin
EC 3.4.22.23: Transferred entry: yeast cysteine proteinase F. Now EC 3.4.21.61, kexin
EC 3.4.22.24: Cathepsin T
EC 3.4.22.25: Glycyl endopeptidase
EC 3.4.22.26: Cancer procoagulant
EC 3.4.22.27: cathepsin S
EC 3.4.22.28: picornain 3C
EC 3.4.22.29: picornain 2A
EC 3.4.22.30: Caricain
EC 3.4.22.31: Ananain
EC 3.4.22.32: Stem bromelain
EC 3.4.22.33: Fruit bromelain
EC 3.4.22.34: Legumain
EC 3.4.22.35: Histolysain
EC 3.4.22.36: caspase-1
EC 3.4.22.37: Gingipain R
EC 3.4.22.38: Cathepsin K
EC 3.4.22.39: adenain
EC 3.4.22.40: bleomycin hydrolase
EC 3.4.22.41: cathepsin F
EC 3.4.22.42: cathepsin O
EC 3.4.22.43: cathepsin V
EC 3.4.22.44: nuclear-inclusion-a endopeptidase
EC 3.4.22.45: helper-component proteinase
EC 3.4.22.46: L-peptidase
EC 3.4.22.47: gingipain K
EC 3.4.22.48: staphopain
EC 3.4.22.49: separase
EC 3.4.22.50: V-cath endopeptidase
EC 3.4.22.51: cruzipain
EC 3.4.22.52: calpain-1
EC 3.4.22.53: calpain-2
EC 3.4.22.54: calpain-3
EC 3.4.22.55: caspase-2
EC 3.4.22.56: caspase-3
EC 3.4.22.57: caspase-4
EC 3.4.22.58: caspase-5
EC 3.4.22.59: caspase-6
EC 3.4.22.60: caspase-7
EC 3.4.22.61: caspase-8
EC 3.4.22.62: caspase-9
EC 3.4.22.63: caspase-10
EC 3.4.22.64: caspase-11
EC 3.4.22.65: peptidase 1 (mite)
EC 3.4.22.66: calicivirin
EC 3.4.22.67: zingipain
EC 3.4.22.68: Ulp1 peptidase
EC 3.4.22.69: SARS coronavirus main proteinase
EC 3.4.22.70: sortase A
EC 3.4.22.71: sortase B

EC 3.4.23 Aspartic endopeptidases

EC 3.4.23.1: pepsin A
EC 3.4.23.2: pepsin B
EC 3.4.23.3: gastricsin
EC 3.4.23.4: chymosin
EC 3.4.23.5: cathepsin D
EC 3.4.23.6: now EC 3.4.23.30 pycnoporopepsin
EC 3.4.23.7: Penicillium janthinellum acid proteinase. Now EC 3.4.23.20, penicillopepsin
EC 3.4.23.8: yeast proteinase A. Now EC 3.4.23.25, saccharopepsin
EC 3.4.23.9: Rhizopus acid proteinase. Now EC 3.4.23.21, rhizopuspepsin
EC 3.4.23.10: Endothia acid proteinase. Now EC 3.4.23.22, endothiapepsin
EC 3.4.23.11: Deleted entry: thyroid aspartic proteinase
EC 3.4.23.12: nepenthesin
EC 3.4.23.13: Deleted entry: Lotus aspartic proteinase
EC 3.4.23.14: Deleted entry: sorghum aspartic proteinase
EC 3.4.23.15: renin
EC 3.4.23.16: HIV-1 retropepsin
EC 3.4.23.17: Pro-opiomelanocortin converting enzyme
EC 3.4.23.18: Aspergillopepsin I
EC 3.4.23.19: Aspergillopepsin II
EC 3.4.23.20: Penicillopepsin
EC 3.4.23.21: Rhizopuspepsin
EC 3.4.23.22: Endothiapepsin
EC 3.4.23.23: Mucorpepsin
EC 3.4.23.24: Candidapepsin
EC 3.4.23.25: Saccharopepsin
EC 3.4.23.26: Rhodotorulapepsin
EC 3.4.23.27: physaropepsin. Now EC 3.4.21.103, physarolisin
EC 3.4.23.28: Acrocylindropepsin
EC 3.4.23.29: Polyporopepsin
EC 3.4.23.30: Pycnoporopepsin
EC 3.4.23.31: Scytalidopepsin A
EC 3.4.23.32: Scytalidopepsin B
EC 3.4.23.33: xanthomonapepsin. Now EC 3.4.21.101, xanthomonalisin
EC 3.4.23.34: Cathepsin e
EC 3.4.23.35: Barrierpepsin
EC 3.4.23.36: Signal peptidase II
EC 3.4.23.37: pseudomonapepsin. Now EC 3.4.21.100, pseudomonalisin
EC 3.4.23.38: plasmepsin I
EC 3.4.23.39: plasmepsin II
EC 3.4.23.40: Phytepsin
EC 3.4.23.41: yapsin 1
EC 3.4.23.42: thermopsin
EC 3.4.23.43: prepilin peptidase
EC 3.4.23.44: nodavirus endopeptidase
EC 3.4.23.45: memapsin 1
EC 3.4.23.46: memapsin 2
EC 3.4.23.47: HIV-2 retropepsin
EC 3.4.23.48: plasminogen activator Pla
EC 3.4.23.49: omptin
EC 3.4.23.50: human endogenous retrovirus K endopeptidase
EC 3.4.23.51: HycI peptidase
EC 3.4.23.52: preflagellin peptidase

EC 3.4.24: Metallopeptidases

EC 3.4.24.1: atrolysin A
EC 3.4.24.2: Deleted entry: Sepia proteinase
EC 3.4.24.3: microbial collagenase
EC 3.4.24.4: now EC 3.4.24.40 serralysin
EC 3.4.24.5: Deleted entry: lens neutral proteinase. Now included with EC 3.4.22.53 (calpain-2) and EC 3.4.25.1 (proteasome endopeptidase complex)
EC 3.4.24.6: leucolysin
EC 3.4.24.7: interstitial collagenase
EC 3.4.24.8: Transferred entry: Achromobacter iophagus collagenase. Now EC 3.4.24.3, microbial collagenase
EC 3.4.24.9: Deleted entry: Trichophyton schoenleinii collagenase
EC 3.4.24.10: Deleted entry: Trichophyton mentagrophytes keratinase
EC 3.4.24.11: neprilysin
EC 3.4.24.12: envelysin
EC 3.4.24.13: IgA-specific metalloendopeptidase
EC 3.4.24.14: procollagen N-endopeptidase
EC 3.4.24.15: thimet oligopeptidase
EC 3.4.24.16: neurolysin
EC 3.4.24.17: stromelysin 1
EC 3.4.24.18: meprin A
EC 3.4.24.19: procollagen C-endopeptidase
EC 3.4.24.20: peptidyl-Lys metalloendopeptidase
EC 3.4.24.21: astacin
EC 3.4.24.22: stromelysin 2
EC 3.4.24.23: matrilysin
EC 3.4.24.24: gelatinase a
EC 3.4.24.25: vibriolysin
EC 3.4.24.26: pseudolysin
EC 3.4.24.27: thermolysin
EC 3.4.24.28: bacillolysin
EC 3.4.24.29: aureolysin
EC 3.4.24.30: coccolysin
EC 3.4.24.31: mycolysin
EC 3.4.24.32: beta-Lytic metalloendopeptidase
EC 3.4.24.33: peptidyl-Asp metalloendopeptidase
EC 3.4.24.34: neutrophil collagenase
EC 3.4.24.35: gelatinase B
EC 3.4.24.36: leishmanolysin
EC 3.4.24.37: saccharolysin
EC 3.4.24.38: gametolysin
EC 3.4.24.39: deuterolysin
EC 3.4.24.40: serralysin
EC 3.4.24.41: atrolysin B
EC 3.4.24.42: atrolysin C
EC 3.4.24.43: atroxase
EC 3.4.24.44: atrolysin E
EC 3.4.24.45: atrolysin F
EC 3.4.24.46: adamalysin
EC 3.4.24.47: horrilysin
EC 3.4.24.48: ruberlysin
EC 3.4.24.49: bothropasin
EC 3.4.24.50: bothrolysin
EC 3.4.24.51: ophiolysin
EC 3.4.24.52: trimerelysin I
EC 3.4.24.53: trimerelysin II
EC 3.4.24.54: mucrolysin
EC 3.4.24.55: pitrilysin
EC 3.4.24.56: insulysin
EC 3.4.24.57: O-sialoglycoprotein endopeptidase
EC 3.4.24.58: russellysin
EC 3.4.24.59: mitochondrial intermediate peptidase
EC 3.4.24.60: dactylysin
EC 3.4.24.61: nardilysin
EC 3.4.24.62: magnolysin
EC 3.4.24.63: meprin B
EC 3.4.24.64: mitochondrial processing peptidase
EC 3.4.24.65: macrophage elastase
EC 3.4.24.66: choriolysin L
EC 3.4.24.67: choriolysin H
EC 3.4.24.68: tentoxilysin
EC 3.4.24.69: bontoxilysin
EC 3.4.24.70: oligopeptidase A
EC 3.4.24.71: endothelin-converting enzyme 1
EC 3.4.24.72: fibrolase
EC 3.4.24.73: jararhagin
EC 3.4.24.74: fragilysin
EC 3.4.24.75: lysostaphin
EC 3.4.24.76: flavastacin
EC 3.4.24.77: snapalysin
EC 3.4.24.78: gpr endopeptidase
EC 3.4.24.79: pappalysin-1
EC 3.4.24.80: membrane-type matrix metalloproteinase-1
EC 3.4.24.81: ADAM10 endopeptidase
EC 3.4.24.82: ADAMTS-4 endopeptidase
EC 3.4.24.83: anthrax lethal factor endopeptidase
EC 3.4.24.84: Ste24 endopeptidase
EC 3.4.24.85: S2P endopeptidase
EC 3.4.24.86: ADAM 17 endopeptidase
EC 3.4.24.87: ADAMTS13 endopeptidase

EC 3.4.25 Threonine endopeptidases

EC 3.4.25.1: proteasome endopeptidase complex
EC 3.4.25.2: HslU—HslV peptidase

EC 3.4.99 Endopeptidases of unknown catalytic mechanism

EC 3.4.99.1: acrocylindricum proteinase. Now EC 3.4.23.28, acrocylindropepsin
EC 3.4.99.2: Deleted entry: agavain
EC 3.4.99.3: Deleted entry: angiotensinase
EC 3.4.99.4: Transferred entry: aspartylendopeptidase. Now EC 3.4.23.12, nepenthesin
EC 3.4.99.5: Transferred entry: Clostridium histolyticum collagenase 2. Now EC 3.4.24.3, microbial collagenase
EC 3.4.99.6: Transferred entry: crayfish low-molecular-weight proteinase. Now EC 3.4.24.21, astacin
EC 3.4.99.7: Deleted entry: euphorbain
EC 3.4.99.8: Deleted entry: Gliocladium proteinase
EC 3.4.99.9: Deleted entry: hurain. Now considered EC 3.4.21.25, cucumisin
EC 3.4.99.10: Transferred entry: insulinase. Now EC 3.4.24.56, insulysin
EC 3.4.99.11: Deleted entry: Streptomyces alkalophilic keratinase
EC 3.4.99.12: Deleted entry: Trichophyton mentagrophytes keratinase
EC 3.4.99.13: Transferred entry: β-lytic proteinase (Mycobacterium sorangium). Now EC 3.4.24.32, β-lytic metalloendopeptidase
EC 3.4.99.14: Deleted entry: mexicanain
EC 3.4.99.15: Deleted entry: Paecilomyces proteinase
EC 3.4.99.16: Deleted entry: Penicillium notatum extracellular proteinase
EC 3.4.99.17: Deleted entry: peptidoglycan endopeptidase
EC 3.4.99.18: Deleted entry: pinguinain
EC 3.4.99.19: Transferred entry: renin. Now EC 3.4.23.15, renin
EC 3.4.99.20: Deleted entry: scopulariopsis proteinase
EC 3.4.99.21: Deleted entry: solanain. Now considered EC 3.4.21.25, cucumisin
EC 3.4.99.22: Transferred entry: staphylokinase. Now EC 3.4.24.29, aureolysin
EC 3.4.99.23: Deleted entry: tabernamontanain. Now considered EC 3.4.21.25, cucumisin
EC 3.4.99.24: Deleted entry: Tenebrio α-proteinase
EC 3.4.99.25: Transferred entry: trametes acid proteinase. Now EC 3.4.23.21, rhizopuspepsin
EC 3.4.99.26: Transferred entry: urokinase. Now EC 3.4.21.68, t-plasminogen activator
EC 3.4.99.27: Deleted entry: Echis carinatus prothrombin-activating proteinase
EC 3.4.99.28: Transferred entry: Oxyuranus scutellatus prothrombin-activating proteinase. Now EC 3.4.21.60, scutelarin
EC 3.4.99.29: Deleted entry: Myxobacter AL-1 proteinase I
EC 3.4.99.30: Transferred entry: Myxobacter AL-1 proteinase II. Now EC 3.4.24.20, peptidyl-Lys metalloendopeptidase
EC 3.4.99.31: Transferred entry: tissue endopeptidase degrading collagenase synthetic substrate. Now EC 3.4.24.15, thimet oligopeptidase
EC 3.4.99.32: Transferred entry: Armillaria mellea neutral proteinase. Now EC 3.4.24.20, peptidyl-Lys metalloendopeptidase
EC 3.4.99.33: Deleted entry: cathepsin R
EC 3.4.99.34: Deleted entry: mytilidase
EC 3.4.99.35: Transferred entry: premurein-leader peptidase. Now EC 3.4.23.36, signal peptidase II
EC 3.4.99.36: Transferred entry: leader peptidase. Now EC 3.4.21.89, signal peptidase I
EC 3.4.99.37: Deleted entry: RecA peptidase
EC 3.4.99.38: Transferred entry: pro-opiomelanotropin-converting proteinase. Now EC 3.4.23.17, pro-opiomelanocortin converting enzyme
EC 3.4.99.39: Deleted entry: pseudomurein endopeptidase
EC 3.4.99.40: Deleted entry: pro-gonadoliberin proteinase
EC 3.4.99.41: Transferred entry: mitochondrial processing peptidase. Now EC 3.4.24.64, mitochondrial processing peptidase
EC 3.4.99.42: Deleted entry: leucyllysine endopeptidase
EC 3.4.99.43: Transferred entry: thermopsin. Now EC 3.4.23.42, thermopsin
EC 3.4.99.44: Transferred entry: pitrilysin. Now EC 3.4.24.55, pitrilysin
EC 3.4.99.45: Transferred entry: insulinase. Now EC 3.4.24.56, insulysin
EC 3.4.99.46: Transferred entry: multicatalytic endopeptidase complex. Now EC 3.4.25.1, proteasome endopeptidase complex

EC 3.5: act on carbon-nitrogen bonds, other than peptide bonds

3.5.1: linear amides

EC 3.5.1.1: asparaginase
EC 3.5.1.2: glutaminase
EC 3.5.1.3: omega-amidase
EC 3.5.1.4: amidase
EC 3.5.1.5: urease
EC 3.5.1.6: beta-ureidopropionase
EC 3.5.1.7: ureidosuccinase
EC 3.5.1.8: formylaspartate deformylase
EC 3.5.1.9: arylformamidase
EC 3.5.1.10: formyltetrahydrofolate deformylase
EC 3.5.1.11: penicillin amidase
EC 3.5.1.12: biotinidase
EC 3.5.1.13: aryl-acylamidase
EC 3.5.1.14: aminoacylase
EC 3.5.1.15: aspartoacylase
EC 3.5.1.16: acetylornithine deacetylase
EC 3.5.1.17: acyl-lysine deacylase
EC 3.5.1.18: succinyl-diaminopimelate desuccinylase
EC 3.5.1.19: nicotinamidase
EC 3.5.1.20: citrullinase
EC 3.5.1.21: N-acetyl-beta-alanine deacetylase
EC 3.5.1.22: pantothenase
EC 3.5.1.23: ceramidase
EC 3.5.1.24: choloylglycine hydrolase
EC 3.5.1.25: N-acetylglucosamine-6-phosphate deacetylase
EC 3.5.1.26: N4-(beta-N-acetylglucosaminyl)-L-asparaginase
EC 3.5.1.27: N-formylmethionylaminoacyl-tRNA deformylase
EC 3.5.1.28: N-acetylmuramoyl-L-alanine amidase
EC 3.5.1.29: 2-(acetamidomethylene)succinate hydrolase
EC 3.5.1.30: 5-aminopentanamidase
EC 3.5.1.31: formylmethionine deformylase
EC 3.5.1.32: hippurate hydrolase
EC 3.5.1.33: N-acetylglucosamine deacetylase
EC 3.5.1.34: acetylhistidine deacetylase
EC 3.5.1.35: D-glutaminase
EC 3.5.1.36: N-methyl-2-oxoglutaramate hydrolase
EC 3.5.1.37: Deleted entry: 4-L-aspartylglycosylamine amidohydrolase. Identical with EC 3.5.1.26 N4-(β-N-acetylglucosaminyl)-L-asparaginase
EC 3.5.1.38: glutamin-(asparagin-)ase
EC 3.5.1.39: alkylamidase
EC 3.5.1.40: acylagmatine amidase
EC 3.5.1.41: chitin deacetylase
EC 3.5.1.42: nicotinamide-nucleotide amidase
EC 3.5.1.43: peptidyl-glutaminase
EC 3.5.1.44: protein-glutamine glutaminase
EC 3.5.1.45: Deleted entry: urease (ATP-hydrolysing). Now listed only as EC 6.3.4.6 urea carboxylase
EC 3.5.1.46: 6-aminohexanoate-dimer hydrolase
EC 3.5.1.47: N-acetyldiaminopimelate deacetylase
EC 3.5.1.48: acetylspermidine deacetylase
EC 3.5.1.49: formamidase
EC 3.5.1.50: pentanamidase
EC 3.5.1.51: 4-acetamidobutyryl-CoA deacetylase
EC 3.5.1.52: peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase
EC 3.5.1.53: N-carbamoylputrescine amidase
EC 3.5.1.54: allophanate hydrolase
EC 3.5.1.55: long-chain-fatty-acyl-glutamate deacylase
EC 3.5.1.56: N,N-dimethylformamidase
EC 3.5.1.57: tryptophanamidase
EC 3.5.1.58: N-benzyloxycarbonylglycine hydrolase
EC 3.5.1.59: N-carbamoylsarcosine amidase
EC 3.5.1.60: N-(long-chain-acyl)ethanolamine deacylase
EC 3.5.1.61: mimosinase
EC 3.5.1.62: acetylputrescine deacetylase
EC 3.5.1.63: 4-acetamidobutyrate deacetylase
EC 3.5.1.64: Nalpha-benzyloxycarbonylleucine hydrolase
EC 3.5.1.65: theanine hydrolase
EC 3.5.1.66: 2-(hydroxymethyl)-3-(acetamidomethylene)succinate hydrolase
EC 3.5.1.67: 4-methyleneglutaminase
EC 3.5.1.68: N-formylglutamate deformylase
EC 3.5.1.69: glycosphingolipid deacylase
EC 3.5.1.70: aculeacin-A deacylase
EC 3.5.1.71: N-feruloylglycine deacylase
EC 3.5.1.72: D-benzoylarginine-4-nitroanilide amidase
EC 3.5.1.73: carnitinamidase
EC 3.5.1.74: chenodeoxycholoyltaurine hydrolase
EC 3.5.1.75: urethanase
EC 3.5.1.76: Arylalkyl acylamidase
EC 3.5.1.77: N-carbamoyl-D-amino acid hydrolase
EC 3.5.1.78: Glutathionylspermidine amidase
EC 3.5.1.79: Phthalyl amidase
EC 3.5.1.80: Deleted entry: N-acetylgalactosamine-6-phosphate deacetylase. Identical to EC 3.5.1.25, N-acetylglucosamine-6-phosphate deacetylase
EC 3.5.1.81: N-acyl-D-amino-acid deacylase
EC 3.5.1.82: N-acyl-D-glutamate deacylase
EC 3.5.1.83: N-acyl-D-aspartate deacylase
EC 3.5.1.84: biuret amidohydrolase
EC 3.5.1.85: (S)-N-acetyl-1-phenylethylamine hydrolase
EC 3.5.1.86: mandelamide amidase
EC 3.5.1.87: N-carbamoyl-L-amino-acid hydrolase
EC 3.5.1.88: peptide deformylase
EC 3.5.1.89: N-acetylglucosaminylphosphatidylinositol deacetylase
EC 3.5.1.90: adenosylcobinamide hydrolase
EC 3.5.1.91: N-substituted formamide deformylase
EC 3.5.1.92: pantetheine hydrolase
EC 3.5.1.93: glutaryl-7-aminocephalosporanic-acid acylase
EC 3.5.1.94: gamma-glutamyl-gamma-aminobutyrate hydrolase
EC 3.5.1.95: N-malonylurea hydrolase
EC 3.5.1.96: succinylglutamate desuccinylase
EC 3.5.1.97: acyl-homoserine-lactone acylase
EC 3.5.1.98: histone deacetylase
EC 3.5.1.99: fatty acid amide hydrolase
EC 3.5.1.100: (R)-amidase
EC 3.5.1.101: L-proline amide hydrolase
EC 3.5.1.102: 2-amino-5-formylamino-6-ribosylaminopyrimidin-4(3H)-one 5'-monophosphate deformylase
EC 3.5.1.103: N-acetyl-1-D-myo-inositol-2-amino-2-deoxy-alpha-D-glucopyranoside deacetylase
EC 3.5.1.104: peptidoglycan-N-acetylglucosamine deacetylase
EC 3.5.1.105: chitin disaccharide deacetylase
EC 3.5.1.106: N-formylmaleamate deformylase
EC 3.5.1.107: maleamate amidohydrolase
EC 3.5.1.108: UDP-3-O-acyl-N-acetylglucosamine deacetylase
EC 3.5.1.109: sphingomyelin deacylase
EC 3.5.1.110: peroxyureidoacrylate/ureidoacrylate amidohydrolase
EC 3.5.1.111: 2-oxoglutaramate amidase
EC 3.5.1.112: 2'-N-acetylparomamine deacetylase
EC 3.5.1.113: 2'''-acetyl-6'''-hydroxyneomycin C deacetylase

3.5.2: cyclic amides

EC 3.5.2.1: barbiturase
EC 3.5.2.2: dihydropyrimidinase
EC 3.5.2.3: dihydroorotase
EC 3.5.2.4: carboxymethylhydantoinase
EC 3.5.2.5: allantoinase
EC 3.5.2.6: beta-lactamase
EC 3.5.2.7: imidazolonepropionase
EC 3.5.2.8: Deleted entry: cephalosporinase. Now included with EC 3.5.2.6 β-lactamase
EC 3.5.2.9: 5-oxoprolinase (ATP-hydrolysing)
EC 3.5.2.10: creatininase
EC 3.5.2.11: L-lysine-lactamase
EC 3.5.2.12: 6-aminohexanoate-cyclic-dimer hydrolase
EC 3.5.2.13: 2,5-dioxopiperazine hydrolase
EC 3.5.2.14: N-methylhydantoinase (ATP-hydrolysing)
EC 3.5.2.15: cyanuric acid amidohydrolase
EC 3.5.2.16: maleimide hydrolase
EC 3.5.2.17: hydroxyisourate hydrolase
EC 3.5.2.18: enamidase
EC 3.5.2.19: streptothricin hydrolase

3.5.3: linear amidines

EC 3.5.3.1: arginase
EC 3.5.3.2: guanidinoacetase
EC 3.5.3.3: creatinase
EC 3.5.3.4: allantoicase
EC 3.5.3.5: formimidoylaspartate deiminase
EC 3.5.3.6: arginine deiminase
EC 3.5.3.7: guanidinobutyrase
EC 3.5.3.8: formimidoylglutamase
EC 3.5.3.9: allantoate deiminase
EC 3.5.3.10: D-arginase
EC 3.5.3.11: agmatinase
EC 3.5.3.12: agmatine deiminase
EC 3.5.3.13: formimidoylglutamate deiminase
EC 3.5.3.14: amidinoaspartase
EC 3.5.3.15: protein-arginine deiminase
EC 3.5.3.16: methylguanidinase
EC 3.5.3.17: Guanidinopropionase
EC 3.5.3.18: dimethylargininase
EC 3.5.3.19: ureidoglycolate hydrolase
EC 3.5.3.20: diguanidinobutanase
EC 3.5.3.21: Methylenediurea deaminase
EC 3.5.3.22: proclavaminate amidinohydrolase
EC 3.5.3.23: N-succinylarginine dihydrolase

3.5.4: cyclic amidines

EC 3.5.4.1: cytosine deaminase
EC 3.5.4.2: adenine deaminase
EC 3.5.4.3: guanine deaminase
EC 3.5.4.4: adenosine deaminase
EC 3.5.4.5: cytidine deaminase
EC 3.5.4.6: AMP deaminase
EC 3.5.4.7: ADP deaminase
EC 3.5.4.8: aminoimidazolase
EC 3.5.4.9: methenyltetrahydrofolate cyclohydrolase
EC 3.5.4.10: IMP cyclohydrolase
EC 3.5.4.11: pterin deaminase
EC 3.5.4.12: dCMP deaminase
EC 3.5.4.13: dCTP deaminase
EC 3.5.4.14: deoxycytidine deaminase
EC 3.5.4.15: guanosine deaminase
EC 3.5.4.16: GTP cyclohydrolase I
EC 3.5.4.17: adenosine-phosphate deaminase
EC 3.5.4.18: ATP deaminase
EC 3.5.4.19: phosphoribosyl-AMP cyclohydrolase
EC 3.5.4.20: pyrithiamine deaminase
EC 3.5.4.21: creatinine deaminase
EC 3.5.4.22: 1-pyrroline-4-hydroxy-2-carboxylate deaminase
EC 3.5.4.23: blasticidin-S deaminase
EC 3.5.4.24: sepiapterin deaminase
EC 3.5.4.25: GTP cyclohydrolase II
EC 3.5.4.26: diaminohydroxyphosphoribosylaminopyrimidine deaminase
EC 3.5.4.27: methenyltetrahydromethanopterin cyclohydrolase
EC 3.5.4.28: S-adenosylhomocysteine deaminase
EC 3.5.4.29: GTP cyclohydrolase IIa
EC 3.5.4.30: dCTP deaminase (dUMP-forming)
EC 3.5.4.31: S-methyl-5'-thioadenosine deaminase
EC 3.5.4.32: 8-oxoguanine deaminase

3.5.5: nitriles

EC 3.5.5.1: nitrilase
EC 3.5.5.2: ricinine nitrilase
EC 3.5.5.3: Transferred entry: cyanate hydrolase. Now EC 4.2.1.104, cyanate hydratase
EC 3.5.5.4: cyanoalanine nitrilase
EC 3.5.5.5: arylacetonitrilase
EC 3.5.5.6: bromoxynil nitrilase
EC 3.5.5.7: aliphatic nitrilase
EC 3.5.5.8: thiocyanate hydrolase

3.5.99: other compounds

EC 3.5.99.1: riboflavinase
EC 3.5.99.2: aminopyrimidine aminohydrolase
EC 3.5.99.3: hydroxydechloroatrazine ethylaminohydrolase
EC 3.5.99.4: N-isopropylammelide isopropylaminohydrolase
EC 3.5.99.5: 2-aminomuconate deaminase
EC 3.5.99.6: glucosamine-6-phosphate deaminase
EC 3.5.99.7: 1-aminocyclopropane-1-carboxylate deaminase
EC 3.5.99.8: 5-nitroanthranilic acid aminohydrolase
EC 3.5.99.9: 2-nitroimidazole nitrohydrolase

EC 3.6: act on acid anhydrides

3.6.1

inorganic diphosphatase
EC 3.6.1.2: trimetaphosphatase
EC 3.6.1.3: adenosinetriphosphatase
EC 3.6.1.4: Deleted entry: adenosinetriphosphatase (Mg-activated). Now included with EC 3.6.1.3 adenosinetriphosphatase
EC 3.6.1.5: apyrase
EC 3.6.1.6: nucleoside-diphosphatase
EC 3.6.1.7: acylphosphatase
EC 3.6.1.8: ATP diphosphatase
EC 3.6.1.9: nucleotide diphosphatase
EC 3.6.1.10: endopolyphosphatase
EC 3.6.1.11: exopolyphosphatase
EC 3.6.1.12: dCTP diphosphatase
EC 3.6.1.13: ADP-ribose diphosphatase
EC 3.6.1.14: adenosine-tetraphosphatase
EC 3.6.1.15: nucleoside-triphosphatase
EC 3.6.1.16: CDP-glycerol diphosphatase
EC 3.6.1.17: bis(5'-nucleosyl)-tetraphosphatase (asymmetrical)
EC 3.6.1.18: FAD diphosphatase
EC 3.6.1.19: nucleoside-triphosphate diphosphatase
EC 3.6.1.20: 5'-acylphosphoadenosine hydrolase
EC 3.6.1.21: ADP-sugar diphosphatase
EC 3.6.1.22: NAD+ diphosphatase
EC 3.6.1.23: dUTP diphosphatase
EC 3.6.1.24: nucleoside phosphoacylhydrolase
EC 3.6.1.25: triphosphatase
EC 3.6.1.26: CDP-diacylglycerol diphosphatase
EC 3.6.1.27: undecaprenyl-diphosphatase
EC 3.6.1.28: thiamine-triphosphatase
EC 3.6.1.29: bis(5'-adenosyl)-triphosphatase
EC 3.6.1.30: m7G(5')pppN diphosphatase
EC 3.6.1.31: phosphoribosyl-ATP diphosphatase
EC 3.6.1.32: Transferred entry: myosin ATPase. Now EC 3.6.4.1, myosin ATPase
EC 3.6.1.33: Transferred entry: dynein ATPase. Now EC 3.6.4.2, dynein ATPase
EC 3.6.1.34: Transferred entry: H⁺-transporting ATP synthase. Now EC 3.6.3.14, H⁺-transporting two-sector ATPase
EC 3.6.1.35: Transferred entry: H⁺-transporting ATPase. Now EC 3.6.3.6, H⁺-exporting ATPase
EC 3.6.1.36: Transferred entry: H⁺/K⁺ exchanging ATPase. Now EC 3.6.3.10, H⁺/K⁺-exchanging ATPase
EC 3.6.1.37: Transferred entry: Na⁺/K⁺ exchanging ATPase. Now EC 3.6.3.9, Na⁺/K⁺-exchanging ATPase
EC 3.6.1.38: Transferred entry: Ca²⁺-transporting ATPase. Now EC 3.6.3.8, Ca²⁺-transporting ATPase
EC 3.6.1.39: thymidine-triphosphatase
EC 3.6.1.40: guanosine-5'-triphosphate,3'-diphosphate diphosphatase
EC 3.6.1.41: bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
EC 3.6.1.42: guanosine-diphosphatase
EC 3.6.1.43: dolichyldiphosphatase
EC 3.6.1.44: oligosaccharide-diphosphodolichol diphosphatase
EC 3.6.1.45: UDP-sugar diphosphatase
EC 3.6.1.46: Transferred entry: heterotrimeric G-protein GTPase. Now EC 3.6.5.1, heterotrimeric G-protein GTPase
EC 3.6.1.47: Transferred entry: small monomeric GTPase. Now EC 3.6.5.2, small monomeric GTPase
EC 3.6.1.48: Transferred entry: protein-synthesizing GTPase. Now EC 3.6.5.3, protein-synthesizing GTPase
EC 3.6.1.49: Transferred entry: signal-recognition-particle GTPase. Now EC 3.6.5.4, signal-recognition-particle GTPase
EC 3.6.1.50: Transferred entry: dynamin GTPase. Now EC 3.6.5.5, dynamin GTPase
EC 3.6.1.51: Transferred entry: tubulin GTPase. Now EC 3.6.5.6, tubulin GTPase
EC 3.6.1.52: diphosphoinositol-polyphosphate diphosphatase
EC 3.6.1.53: Mn2+-dependent ADP-ribose/CDP-alcohol diphosphatase
EC 3.6.1.54: UDP-2,3-diacylglucosamine diphosphatase
EC 3.6.1.55: 8-oxo-dGTP diphosphatase
EC 3.6.1.56: 2-hydroxy-dATP diphosphatase
EC 3.6.1.57: UDP-2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose hydrolase
EC 3.6.1.58: 8-oxo-dGDP phosphatase
EC 3.6.1.59: m7GpppX diphosphatase
EC 3.6.1.60: diadenosine hexaphosphate hydrolase (AMP-forming)
EC 3.6.1.61: diadenosine hexaphosphate hydrolase (ATP-forming)
EC 3.6.1.62: m7GpppN-mRNA hydrolase
EC 3.6.1.63: alpha-D-ribose 1-methylphosphonate 5-triphosphate diphosphatase

3.6.2

EC 3.6.2.1: adenylylsulfatase
EC 3.6.2.2: phosphoadenylylsulfatase

3.6.3

EC 3.6.3.1: phospholipid-translocating ATPase
EC 3.6.3.2: Mg2+-importing ATPase
EC 3.6.3.3: Cd2+-exporting ATPase
EC 3.6.3.4: Cu2+-exporting ATPase
EC 3.6.3.5: Zn2+-exporting ATPase
EC 3.6.3.6: H+-exporting ATPase
EC 3.6.3.7: Na+-exporting ATPase
EC 3.6.3.8: Ca2+-transporting ATPase
EC 3.6.3.9: Na+/K+-exchanging ATPase
EC 3.6.3.10: H+/K+-exchanging ATPase
EC 3.6.3.11: Cl-transporting ATPase
EC 3.6.3.12: K+-transporting ATPase
EC 3.6.3.14: H+-transporting two-sector ATPase
EC 3.6.3.15: Na+-transporting two-sector ATPase
EC 3.6.3.16: arsenite-transporting ATPase
EC 3.6.3.17: monosaccharide-transporting ATPase
EC 3.6.3.18: oligosaccharide-transporting ATPase
EC 3.6.3.19: maltose-transporting ATPase
EC 3.6.3.20: glycerol-3-phosphate-transporting ATPase
EC 3.6.3.21: polar-amino-acid-transporting ATPase
EC 3.6.3.22: nonpolar-amino-acid-transporting ATPase
EC 3.6.3.23: oligopeptide-transporting ATPase
EC 3.6.3.24: nickel-transporting ATPase
EC 3.6.3.25: sulfate-transporting ATPase
EC 3.6.3.26: nitrate-transporting ATPase
EC 3.6.3.27: phosphate-transporting ATPase
EC 3.6.3.28: phosphonate-transporting ATPase
EC 3.6.3.29: molybdate-transporting ATPase
EC 3.6.3.30: Fe3+-transporting ATPase
EC 3.6.3.31: polyamine-transporting ATPase
EC 3.6.3.32: quaternary-amine-transporting ATPase
EC 3.6.3.33: vitamin B12-transporting ATPase
EC 3.6.3.34: iron-chelate-transporting ATPase
EC 3.6.3.35: manganese-transporting ATPase
EC 3.6.3.36: taurine-transporting ATPase
EC 3.6.3.37: guanine-transporting ATPase
EC 3.6.3.38: capsular-polysaccharide-transporting ATPase
EC 3.6.3.39: lipopolysaccharide-transporting ATPase
EC 3.6.3.40: teichoic-acid-transporting ATPase
EC 3.6.3.41: heme-transporting ATPase
EC 3.6.3.42: beta-glucan-transporting ATPase
EC 3.6.3.43: peptide-transporting ATPase
EC 3.6.3.44: xenobiotic-transporting ATPase
EC 3.6.3.45: steroid-transporting ATPase
EC 3.6.3.46: cadmium-transporting ATPase
EC 3.6.3.47: fatty-acyl-CoA-transporting ATPase
EC 3.6.3.48: alpha-factor-transporting ATPase
EC 3.6.3.49: channel-conductance-controlling ATPase
EC 3.6.3.50: protein-secreting ATPase
EC 3.6.3.51: mitochondrial protein-transporting ATPase
EC 3.6.3.52: chloroplast protein-transporting ATPase
EC 3.6.3.53: Ag+-exporting ATPase

3.6.4

EC 3.6.4.1: myosin ATPase
EC 3.6.4.2: dynein ATPase
EC 3.6.4.3: microtubule-severing ATPase
EC 3.6.4.4: plus-end-directed kinesin ATPase
EC 3.6.4.5: minus-end-directed kinesin ATPase
EC 3.6.4.6: vesicle-fusing ATPase
EC 3.6.4.7: peroxisome-assembly ATPase
EC 3.6.4.8: proteasome ATPase
EC 3.6.4.9: chaperonin ATPase
EC 3.6.4.10: non-chaperonin molecular chaperone ATPase
EC 3.6.4.11: nucleoplasmin ATPase
EC 3.6.4.12: DNA helicase
EC 3.6.4.13: RNA helicase

3.6.5

EC 3.6.5.1: heterotrimeric G-protein GTPase
EC 3.6.5.2: small monomeric GTPase
EC 3.6.5.3: protein-synthesizing GTPase
EC 3.6.5.4: signal-recognition-particle GTPase
EC 3.6.5.5: dynamin GTPase
EC 3.6.5.6: tubulin GTPase

EC 3.7: act on carbon-carbon bonds

EC 3.7.1.1: oxaloacetase
EC 3.7.1.2: fumarylacetoacetase
EC 3.7.1.3: kynureninase
EC 3.7.1.4: phloretin hydrolase
EC 3.7.1.5: acylpyruvate hydrolase
EC 3.7.1.6: acetylpyruvate hydrolase
EC 3.7.1.7: beta-diketone hydrolase
EC 3.7.1.8: 2,6-dioxo-6-phenylhexa-3-enoate hydrolase
EC 3.7.1.9: 2-hydroxymuconate-semialdehyde hydrolase
EC 3.7.1.10: cyclohexane-1,3-dione hydrolase
EC 3.7.1.11: cyclohexane-1,2-dione hydrolase
EC 3.7.1.12: cobalt-precorrin 5A hydrolase
EC 3.7.1.13: 2-hydroxy-6-oxo-6-(2-aminophenyl)hexa-2,4-dienoate hydrolase
EC 3.7.1.14: 2-hydroxy-6-oxonona-2,4-dienedioate hydrolase
EC 3.7.1.15: (+)-caryolan-1-ol synthase
EC 3.7.1.16: oxepin-CoA hydrolase
EC 3.7.1.17: 4,5:9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oate hydrolase
EC 3.7.1.18: 6-oxocamphor hydrolase
EC 3.7.1.19: 2,6-dihydroxypseudooxynicotine hydrolase
EC 3.7.1.20: 3-fumarylpyruvate hydrolase

EC 3.8: act on halide bonds

EC 3.8.1.1: alkylhalidase
EC 3.8.1.2: 2-haloacid dehalogenase
EC 3.8.1.3: haloacetate dehalogenase
EC 3.8.1.4: thyroxine deiodinase
EC 3.8.1.5: haloalkane dehalogenase
EC 3.8.1.6: 4-chlorobenzoate dehalogenase
EC 3.8.1.7: 4-chlorobenzoyl-CoA dehalogenase
EC 3.8.1.8: atrazine chlorohydrolase
EC 3.8.1.9: (R)-2-haloacid dehalogenase
EC 3.8.1.10: 2-haloacid dehalogenase (configuration-inverting)
EC 3.8.1.11: 2-haloacid dehalogenase (configuration-retaining)

EC 3.8.2.1: Transferred entry: di-isopropyl-fluorophosphatase. Now EC 3.1.8.2, diisopropyl-fluorophosphatase

EC 3.9: act on phosphorus-nitrogen bonds

EC 3.9.1.1: phosphoamidase

EC 3.10: act on sulfur-nitrogen bonds

EC 3.10.1.1: N-sulfoglucosamine sulfohydrolase
EC 3.10.1.2: cyclamate sulfohydrolase

EC 3.11: act on carbon-phosphorus bonds

EC 3.11.1.1: phosphonoacetaldehyde hydrolase
EC 3.11.1.2: phosphonoacetate hydrolase
EC 3.11.1.3: phosphonopyruvate hydrolase

EC 3.12: act on sulfur-sulfur bonds

EC 3.12.1.1: trithionate hydrolase

EC 3.13: act on carbon-sulfur bonds

EC 3.13.1.1: UDP-sulfoquinovose synthase
EC 3.13.1.2: Deleted entry: 5-deoxyribos-5-ylhomocysteinase. The activity is most probably attributable to EC 4.4.1.21, S-ribosylhomocysteine lyase
EC 3.13.1.3: 2'-hydroxybiphenyl-2-sulfinate desulfinase

Proteins: enzymes

Activity

Regulation

Classification

Types

EC1 Oxidoreductases(list)
EC2 Transferases(list)
EC3 Hydrolases(list)
EC4 Lyases(list)
EC5 Isomerases(list)
EC6 Ligases(list)

By EC number:

Hydrolase: esterases (EC 3.1)

3.1.1: Carboxylic
ester hydrolases

Lipase

Phospholipase
- A1
- A2
- B

3.1.2: Thioesterase

3.1.3: Phosphatase

3.1.4: Phosphodiesterase

3.1.6: Sulfatase

Nuclease (includes
deoxyribonuclease and
ribonuclease)

3.1.11-16: Exonuclease

Exodeoxyribonuclease	RecBCD

Exoribonuclease	Oligonucleotidase

3.1.21-31: Endonuclease

Endodeoxyribonuclease	Deoxyribonuclease I Deoxyribonuclease II Deoxyribonuclease IV Restriction enzyme UvrABC endonuclease

Endoribonuclease	RNase III RNase H 1 2A 2B 2C RNase P RNase A 1 2 3 4/5 RNase T1 RNA-induced silencing complex

either deoxy- or ribo-	Aspergillus nuclease S1 Micrococcal nuclease

By EC number:

Hydrolase: sugar hydrolases (EC 3.2)

3.2.1: Glycoside hydrolases

Disaccharidase	Sucrase/Sucrase-isomaltase/Invertase Maltase Trehalase Lactase

Glucosidases	Cellulase Alpha-glucosidase Acid Neutral AB Neutral C Beta-glucosidase cytosolic Debranching enzyme

Other	Amylase Alpha-amylase Chitinase Lysozyme Neuraminidase NEU1 NEU2 NEU3 NEU4 Bacterial neuraminidase Viral neuraminidase Galactosidases Alpha Beta alpha-Mannosidase Glucuronidase Hyaluronidase Pullulanase Glucosylceramidase lysosomal non-lysosomal Galactosylceramidase Alpha-N-acetylgalactosaminidase NAGA Alpha-N-acetylglucosaminidase Fucosidase Hexosaminidase HEXA HEXB Iduronidase Maltase-glucoamylase Heparanase HPSE2

3.2.2: Hydrolysing
N-Glycosyl compounds

DNA glycosylases: Oxoguanine glycosylase

By EC number:

Hydrolases: ether bond (EC 3.3)

3.3.1

Adenosylhomocysteinase

3.3.2

By EC number:

Hydrolase: proteases (EC 3.4)

3.4.11-19: Exopeptidase

3.4.11	Aminopeptidase Alanine Arginyl Aspartyl Cystinyl Leucyl Glutamyl Methionyl 1 2 O

3.4.13	Dipeptidase 1 2 3

3.4.14	Dipeptidyl peptidase Cathepsin C Dipeptidyl peptidase-4 Tripeptidyl peptidase Tripeptidyl peptidase I Tripeptidyl peptidase II

3.4.15	Angiotensin-converting enzyme

3.4.16	Serine type carboxypeptidases: Cathepsin A DD-transpeptidase

3.4.17	Metalloexopeptidases Carboxypeptidase A A2 B C E Glutamate II

Other/ungrouped	Metalloexopeptidase

3.4.21-25: Endopeptidase

Other/ungrouped: Amyloid precursor protein secretase

3.4.99: Unknown

Staphylokinase

By EC number:

Hydrolases: carbon-nitrogen non-peptide (EC 3.5)

3.5.1: Linear amides /
Amidohydrolases

3.5.2: Cyclic amides/
Amidohydrolases

3.5.3: Linear amidines/
Ureohydrolases

3.5.4: Cyclic amidines/
Aminohydrolases

3.5.5: Nitriles/
Aminohydrolases

Nitrilase

3.5.99: Other

By EC number:

Hydrolases: acid anhydride hydrolases (EC 3.6)

3.6.1

3.6.2

3.6.3-4: ATPase

3.6.3

Cu++ (3.6.3.4)	Menkes/ATP7A Wilson/ATP7B

Ca+ (3.6.3.8)	SERCA ATP2A1 ATP2A2 ATP2A3 Plasma membrane ATP2B1 ATP2B2 ATP2B3 ATP2B4 SPCA ATP2C1 ATP2C2

Na+/K+ (3.6.3.9)	ATP1A1 ATP1A2 ATP1A3 ATP1A4 ATP1B1 ATP1B2 ATP1B3 ATP1B4

H+/K+ (3.6.3.10)	ATP4A

Other P-type ATPase	ATP8B1 ATP10A ATP11B ATP12A ATP13A2 ATP13A3

3.6.4

3.6.5: GTPase

3.6.5.1: Heterotrimeric G protein	G_αs G_αi GNAI1 GNAI2 GNAI3 G_αq/11 GNAQ GNA11 G_α12/13 GNA12 GNA13 Transducin GNAT1 GNAT2

3.6.5.2: Small GTPase > Ras superfamily	Rho family of GTPases: Cdc42 CDC42 TC10 TCL RhoUV RhoU RhoV Rac Rac1 2 3 RhoG RhoBTB 1 2 RhoH Rho A B C Rnd 1 2 3 RhoDF RhoF RhoD other: Ras HRAS KRAS NRAS Rab RAB23 RAB27 Arf ARF6 SAR1B ARL13B ARL6 Ran Rheb Rap RGK

3.6.5.3: Protein-synthesizing GTPase	Prokaryotic IF-2 EF-Tu EF-G Eukaryotic

3.6.5.5-6: Polymerization motors	Dynamin Tubulin

By EC number:

Hydrolases: carbon-carbon (EC 3.7)

3.7.1

By EC number:

List of EC numbers (EC 3)